A method based on electrostatic resonance for measuring Young’s modulus of microfibers is developed in this paper. The resonance frequencies of cantilevered microfibers are directly determined by using an optical microscope, rather than an expensive high-precision microsensors or laser Doppler vibrators used in previous studies. The Young’s moduli of microfibers are derived from Euler-Bernoulli beam theory by using measured resonant frequencies. This method is beneficial to improve the comprehensive experimental ability of undergraduates, stimulate their interest in learning, enhance their awareness of innovation, and help them to consciously apply physical principles and measurement methods to the new areas of science and technology.