The centrifugal technique is an experimental technique, which separates particulate matter according to the difference of the sedimentation coefficient by centrifugal force. However, numerous approximate treatments exist in the centrifugal separation theory, such as ignoring Coriolis force, treating the centrifugal process of particulate matter as a uniform motion. In this research, a rigorous kinetic analysis for the centrifugal process of particulate matter by differential centrifugation method and sedimentation velocity method is carried out according to the basic principles of Newtonian mechanics. Combining with numerical calculation of the relevant parameters of bovine serum albumin (BSA), this research focuses on the rationality of the uniform motion treatment.