Tracker is an opensource software for video analysis and modeling. Inducting reasonably Tracker in physics experiment teaching to optimization design can not only enhance the digital construction of experimental courses, but also stimulate students’ interest in exploring experiments. In this paper, the application of Tracker software in distance measurement, trajectory tracking, and angle measurement in physical experiments is explored by combining three experiments: laser wavelength measurement by Young’s doubleslit interferometry, gravitational acceleration measurement by single pendulum and the influence of pendulum angle θ of simple pendulum on measurement results of gravitational acceleration. The experimental results show that the Tracker software can improve the measurement accuracy of the adjacent bright fringe spacing Δx in the Young’s doubleslit interference experiment, and more accurately measure the period of the single pendulum in the gravitational acceleration measurement experiment of single pendulum, and also has good measurement results for exploring the influence of the pendulum angle θ on the measurement results in the experiment of single pendulum measurement of gravity acceleration experiment. Therefore, the introduction of the experiment can effectively reduce the parallax caused by human eye observation and the measurement error in the experiment, and can accurately measure the angle and other physical quantities that are difficult to accurately measure in the actual scene.